Electronic Theses and Dissertation

Bunga rosela (Hibiscus sabdariffa L.) merupakan tanaman yang memiliki kandungan antosianin yang dapat berperan sebagai senyawa antioksidan alami. Antioksidan ialah senyawa yang dapat menangkal radikal bebas dan mampu menghambat proses penuaan dini. Tujuan penelitian ini adalah untuk membuat formulasi sediaan gel serum dari ekstrak etanol kelopak bunga rosela dan efek anti-aging terhadap hewan uji. Ekstrak etanol kelopak bunga rosela diformulasikan menjadi sediaan gel serum serta diuji aktivitas antioksidan dari ekstrak menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Hasil uji aktivitas antioksidan memiliki nilai IC50 yaitu 5,075 ppm yang tergolong aktivitas antioksidan sangat kuat. Hasil evaluasi sediaan gel serum ekstrak etanol kelopak bunga rosela yang meliputi uji organoleptik, uji viskositas, uji pH, uji daya sebar, uji daya lekat, uji stabilitas semua telah memenuhi persyaratan. Hasil uji iritasi sediaan tidak menunjukkan adanya tanda-tanda iritasi sehingga sediaan gel serum ekstrak etanol kelopak bunga rosela relatif aman digunakan. Uji aktivitas terhadap hewan coba menunjukkan bahwa gel serum ekstrak etanol kelopak bunga rosela 0,010% mampu meningkatkan kelembapan 11,6% (normal), kolagen 60% (penurunan jaringan kolagen) dan elastisitas 38,6% (elastisitas lemah).
Kata kunci: Anti-aging, DPPH (2,2-diphenyl-1-picrylhdrazyl), ekstrak bunga rosela, gel serum.

Roselle flower (Hibiscus sabdariffa L.) is a plant that contains anthocyanins, which can act as natural antioxidant. Antioxidants are that can counteract free radicals and can inhibit the premature aging process. The purpose of this study was to formulate a serum gel preparation from the ethanolic extract of roselle petals and its anti-aging effect on test animals. The ethanolic extract of roselle petals was formulated into a serum gel preparation and the antioxidant activity of the extract was tested using the DPPH method (2,2-diphenyl-1-picrylhydrazyl). The results of the antioxidant activity test have an IC50 value of 5.075 ppm, which is classified as very strong antioxidant activity. The results of the evaluation of the serum gel preparation of roselle petals ethanol extract, which included organoleptic tests, viscosity tests, pH tests, dispersion tests, adhesion tests, stability tests all met the requirements. The results of the irritation test of the preparation did not show any signs of irritation so that the serum gel preparation of ethanolic extract of roselle petals was relatively safe to use. Activity tests on experimental animals showed that serum gel of 0.010% roselle petals ethanol extract was able to increase humidity 11.6 % (normal), collagen, 60% (decrease in collagen tissue) and 38.6% elasticity (weak elasticity). Keywords: Anti-aging, DPPH (2,2-diphenyl-1-picrylhydrazyl), roselle flower extract, serum gel.