Aquilaria malaccensis is one of the herbs that have antibacterial activity. This study has been conducted to determine the antibacterial activity of n-hexane and ethanol A. Malaccensis leaves extracts against Escherichia coli and Staphylococcus aureus. The method that was used in this study was A. malaccensis leaves extraction using stage maceration. A. malaccensis leaves extracts were evaluated for characterization of extract, phytochemical test, GC-MS analysis, and antibacterial activity by well diffusion method. The concentration of the extract was 5; 10; 15; 20; and 30%. A. malaccensis leaves simplicial characterization contains water content value of 6,5%, water-soluble extract content of 13,13%, ethanol-soluble extract content of 10,83%, and total ash content of 6,16%. n-Hexane extract from A. malaccensis leaves contains a water content of 18,16%, water-soluble extract content of 6,66%, ethanol-soluble extract content of 35,83%, and total ash content of 0,66%. Ethanol extract from A. malaccensis leaves contains a water content value of 13,5%, water-soluble extract content of 58,33%, ethanol-soluble extract content of 75,83%, and total ash content of 9%. The phytochemical analysis showed that A. malaccensi leaves simplicial ethanol extract contained flavonoid, alkaloid compounds, saponins, tannins, steroids, tritepenoids and n-hexane contains steroids, triterpenoids. GC-MS analysis of n-hexane extract contains compounds Squalene (14,75%), DOCASANE (8,85%), ICOSANE (7,01%) and METHYL (9Z,12Z,15Z)-9,12,15-TETRAMETHYL-2-HEXADECE-1-OL (4,23%). GC-MS analysis of ethanol extract contains Squalene (12,39%), Phytol (11,99%), Heneicosane (6,01%), 9,12,15-Octadecatrienoic acid, methyl ester (5,85%), 1,2-Benzenedicarboxylic acid, mono (2-Ethylhexyl) ester (5,57%), beta. –Sitosterol (5,43%), ICOSANE (4,57%), Vitamin E (4,22%), Hexadecanoic acid, and methyl ester (4,02%). The results of antibacterial activity showed that A. malaccensis leaves n-hexane extract exhibited no activity against E. coli and S. Aureus, but A. malaccensis leaves ethanol extract with a concentration of 5; 10; 15; 20 and 30% against E. coli shown inhibition zone by 6,09; 6,72; 7,78; 8,65; and 9,63 mm respectively while against S. aureus shown inhibition zone by 10,37; 10,44; 11,63; 12,21; and 13,34 mm respectively.