The research aimed to find out the effectivity of rhizobacteria application in germination growth and systemic resistance of the red chili pepper againts to the Colletotrichum spp. pathogen. The research was conducted in the Laboratory of Seed Science and Technology, Laboratory of Plant Disease of the Faculty of Agriculture of Universitas Syiah Kuala, Darussalam, Banda Aceh from January to February 2021. This research used a Completely Randomised Design with a non-factorial pattern with 10 levels of treatment and 3 replications. The result shows that the seed treatment with rhizobacteria plant growth booster proves a very real significant of maximum growth potential, germination rate, growth simultaneity, relative growth speed, T50, plant height at 15 and 30 days after transplanting (DAT), stem base diameter at 30 DAT, number of leaves at 15 and 30 DAT, percentage of disease incidence and peroxidase enzyme activity, as well as has a real significant towards the Vigor Index but has no real significant towards plant height at 0 DAT, stem base diameter at 0 DAT, stem base diameter at 15 DAT, and number of leaves at 0 DAT.