After the emergence of a new viral disease in 2019 which later became the COVID-19 pandemic, the demands for the work of health workers, in this case especially nurses, have been increasing due to the large number of patients who contracted the virus. As a result, nurses have been experiencing stress at work. Stress at work for a nurse is a pressure that comes from the work environment, organizations and individuals that require nurses to be able to make adjustments. The objective of this study was to identify the factors associated with stress at work of nurses during covid-19 pandemic in Regional Public Hospital of Meuraxa in Banda Aceh. This quantitative study was conducted by means of a retrospective study approach. A number of 65 nurses who have ever treated covid-19 patients were chosen as the respondents by using a total sampling technique. The data were collected by distributing a questionnaire assessing factors associated with stress and the scale of psychometric evaluation of healthcare workers stress related to caring for patients with a highly here infectious disease. The data were the analyzed with Chi-Square test. The results showed that there was a relation between environmental (with p-value of 0.025), organizational (with p-value of 0.004), and individual (with p-value of 0.001) factors as well as all factors in general (with p-value of 0.014) and the stress at work of nurses during covid-19 pandemic in Regional Public Hospital of Meuraxa. Hence, it is suggested that the management of the hospital always evaluate the nurses’ work environment in order to create a healthy workplace for them, so that their physical and psychological health of the nurses can be maintained.