Herbal medicine can be an alternative option that is safer to use. moringa has been used as medicine because of its various benefits, one of which is an antibacterial. This study aims to determine the activity of moringa leaf which is extracted by using the stratification maceration with n-hexane, ethyl acetate, and ethanol 96% as an antibacterial against Escherichia coli by in vitro. This study is an experimental laboratory with 6 treatments and 3 replications. The method used is Kirby-Bauer agar diffusion with 5 concentrations, 0%, 10%, 20%, 40%, 60%, and chloramphenicol disc 30 μg as the positive control. The extent of the inhibition zone was obtained by measuring the diameter of the clear zone around the paper disc. The data were analyzed descriptively and presented with tables. The results showed that the n-hexane extract of moringa leaf does not produce any inhibition zone, while the ethyl acetate extract of moringa leaf has an inhibition zone at a concentration of 60% with an average of 10,8±1,2 mm. Ethanol extract of moringa leaf showed inhibition zone at concentrations 10%, 20%, 40%, and 60% with an average inhibition zone 10,24±0,7 mm, 11,25±0,7 mm, 12,87±0,8 mm, and 14,42±0,6 mm. The conclusion is, the ethyl acetate and ethanol 96% of moringa leaf extract showed antibacterial activity against Escherichia coli, while n-hexane extract of moringa leaf did not show antibacterial activity against Escherichia coli.
Keywords: N-hexane, ethyl acetate, ethanol 96%, maceration, phytocemical